所以，这篇文章详细综述了一个经典的single-cell RNA-seq分析流程，包括数据预处理（质控，标准化，数据校正，特征选择和数据降维）和细胞/基因水平的下游分析。

Current scRNA-seq protocols involve isolating single cells and their RNA, and then following the same steps as bulk RNA-seq: reverse transcription (RT), amplification, library generation and …

Single‐cell RNA sequencing (scRNA‐seq) technology has become the state‐of‐the‐art approach for unravelling the heterogeneity and complexity of RNA transcripts within individual cells, as …

Explore the basics of single cell RNA-sequencing in this technology overview article, plus find helpful links for getting started with sample prep, library construction, and data analysis.

Apr 26, 2023 · 单细胞 RNA 测序（Single cell RNA sequencing，scRNA-seq）是一种在单细胞水平上利用 RNA 测序对特细胞群体进行基因表达谱定量的高通量实验技术。 待测组织经过单细 …

Apr 1, 2025 · In the field of single-cell RNA sequencing (scRNA-seq), emerging technologies such as multi-omics integration and real-time single-cell analysis are opening up exciting new …

6 days ago · CytoTRACE 2 is an interpretable deep learning framework that leverages single-cell RNA sequencing data to predict absolute developmental potential across datasets.

Jul 23, 2025 · Single-cell ribonucleic acid sequencing (scRNA-seq) has revolutionized our understanding of complex biological systems by allowing the analysis of genetic information at …

Nov 23, 2023 · 单细胞转录组分析通常指的 单细胞 RNA 测序（single-cell RNA-seq，简称 scRNA-seq） 数据分析。 scRNA-seq 是一种研究单细胞的基因表达模式的方法，能够揭示细胞的异质 …

Different protocols and currently functional single-cell databases were also covered. This review highlights different tools for the analysis of scRNA-seq and their methodologies, emphasizing …