The metaphors we used to describe DNA make it sound almost sacred. Proteins, too, are revered. They are the “workhorses of the cell.” They make things happen. RNA, by contrast, has not been so exalted ...

Years of experiments and databases filled with RNA-seq results belie the simple reality that, until fairly recently, it was impossible to analyze RNA directly. The RNA-seq studies performed with ...

Starting from whole plant tissues and dissociating cells (protoplasts) or nuclei from the tissue, RNA extraction, cDNA synthesis by reverse transcription, library preparation followed by sequencing, ...